Synthesis

DNA Synthesis

DNA Synthesis
  • Synthesis of modified and labeled oligonucleotides for research, diagnosis and other applications.
  • Advice on oligonucleotide sequence, modification and experimental design.
  • Two-step trityl-ON/trityl-OFF HPLC purification of DNA oligonucleotides, offering much higher purity than typically offered by commercial DNA synthesis services.
  • Option for gel purification using super-long gel purification rigs.
  • Characterisation of oligonucleotides: UV/vis absorption spectrum, HPLC traces, DNA amount. High-resolution mass-spectra on request via SIRCAMS facilities in Edinburgh (1 amu accuracy).

High Pressure Facilities

High Pressure Facilities

In the Centre for Science at Extreme Conditions:

  • high pressure single crystal X-ray diffraction (up to 10 GPa (ca 100,000 times atmospheric).
  • Diamond anvil cells containment for high pressure, single crystal work for Xray and neutron studies.
  • CuBe contained, variable, high pressure SQUID measurements (to 10 Gbar).

Biophysical Characterisation Facility

Biophysical Characterisation Facility

A service providing 11 networked ÅKTA liquid chromatography units, of various configurations and levels of automation, an extensive core column and chromatography resin library, HT robotic cloning and screening facilities, growth/incubation areas, a tissue culture suite and a set of core wet labs with access to automated cell lysis, centrifugation, and analysis instrumentation.

Protein Purification Facility

Protein Purification Facility

A service providing 11 networked ÅKTA liquid chromatography units, of various configurations and levels of automation, an extensive core column and chromatography resin library, HT robotic cloning and screening facilities, growth/incubation areas, a tissue culture suite and a set of core wet labs with access to automated cell lysis, centrifugation, and analysis instrumentation.

Molecular Biology Service

Molecular Biology Service

This service offers two complementary technologies: large scale fermentation and general molecular biology cloning. The overall supervisor is David Dryden (50 4735).

  • Fermentation comprises three BIOFLO4500 fermenters in which we may grow recombinant and non-recombinant micro organisms to a 10 litre scale plus two smaller fermenters. We have expertise in growing E. coli, Saccharomyces cerevisiae and Pichia pastoris although the growth of any category 1 (medically harmless) micro organism with simple growth requirements is possible. Cell harvesting is part of the service with our large volume centrifuge, with the end product provided either as cell pellet or supernatant.
  • General molecular biology services include construction of overexpression plasmids, gel electrophoresis, DNA preparation, PCR and long term storage in -80oC freezers. Advice on procedures can also be given.

Syntheses of inorganic and organometallic complexes

Syntheses of inorganic and organometallic complexes

Syntheses of inorganic and organometallic complexes

  • Individual, dedicated wide (1.5m) fume-hoods with high-vacuum Schlenk equipment (sub 1 ppm O2/H2O).
  • Full capabilities for organic synthesis at up to 0.5 kg scales.
  • Wide range of glassware for manipulation of complexes and for variable temperature reactions. Capabilities for sample sealing under variety of gases or low pressures.
  • Solvent drying towers for synthetic chemistry.
  • Glove-boxes for sample preparation and manipulation in inert atmosphere (scrubbed, high-purity N2 atmosphere, sub 1 ppm O2/H2O).
  • High vacuum sublimation line (10-6 mbar, 400 C).
  • High vacuum, greaseless gas transfer reaction line, and equipment for a variety of gas reactions at different temperatures and pressures.
  • Box furnaces to 1000 C.
  • Microwave reactors in various configurations, including CEM Discover microwave reactor.
  • Cells and ovens for high pressure and hydrothermal synthesis.